Structure and function of proteins. Principles of thermodynamics. Kinetic and enzymatic catalysis. Structure and function of carbohydrates, lipids and nucleic acids. The metabolism of proteins, carbohydrates, lipids and nucleic acids. Spectrophotometric and Electrophoretic techniques.
1) Garrett & Grisham “Biochimica” V ed.; Piccin Ed.
2) Campbell Biochimica” V ed.; EDISES Ed.
3) Voet, "Fondamenti di Biochimica" iV ed.; Zanichelli
Learning Objectives
The course aims to train the student in relation to the general principles of Biochemistry. This course provides knowledge about the structure and function of biological macromolecules, methods and techniques used for their study, metabolism and metabolic regulation.
Prerequisites
The course requires knowledge of general chemistry, organic and basic notions of biology.
Teaching Methods
Lectures, carried out in dual mode (in presence and remotely) according to rectoral indications in the Covid-19 pandemic period. Teaching materials: Slides of the lessons available in Pdf. Tools to support teaching: Video projector, PC, Moodle e-learning platform.
Further information
student reception hours
Prof. P. Cirri: tuesday 14,00-17,00
Prof.ssa A. Caselli:
monday 15,30
Location:
Dipartimento di Scienze Biomediche Sperimentali e Cliniche "Mario Serio", viale Morgagni 50, 50134 Firenze.
Type of Assessment
The exam consists of an ORAL interview (in presence or at a distance according to the rector's indications relating to the pandemic period) and concerns the topics of the program. the teacher will articulate specific questions aimed at verifying the preparation on the subject and the knowledge acquired throughout the course, the ability to discursively organize knowledge, the ability to connect between different topics, as well as the competence in the use of the appropriate scientific lexicon and the effectiveness of the exhibition. The final grade results from all these contributions considered important indicators of the adequacy of the level of learning achieved and the achievement of the learning outcomes envisaged by the course.
Course program
General principles of biochemistry. The amino acids. The peptide bond. Structure of proteins. Hemoglobin. Enzymes and enzyme kinetics. Regulation of enzyme activity. Inhibition of enzyme activity. Mechanism of enzyme action. Allosteric enzymes. Biological macromolecules: lipids and lipoproteins, carbohydrates and glycoproteins, nucleic acids. General principles of metabolism. Glycolysis. Pentose-phosphate cycle. Citric acid cycle. Respiratory chain and oxidative phosphorylation. Glycogen synthesis and glycogen-lysis. Mechanisms of hormonal regulation of glucose metabolism. Activation, transport and oxidation of fatty acids. Chetonic bodies. Biosynthesis of fatty acids. Cholesterol metabolism. Mechanisms of hormonal regulation of lipid metabolism. Degradation of amino acids: urea cycle and fate of carbon skeleton. General aspects of pyrimidine and purine bases metabolism. Molecules derived from amino acids. DNA duplication. Transcription and translation. Integration and hormonal regulation of metabolism in mammals.
Applied Biochemistry: spectrophotometry: principles and practical applications. assay methods of the enzymes. Determination of the main kinetic parameters. Electrophoretic techniques. Analysis of protein profiles. Mass Spectrometry.